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UVA-TEA IS EFFECTIVE AGAINST FIVE MAJOR CARCINOMA CELLS LINES
Research of Polish Academy of Science( Ryszard Amarrowicz, Prof Phd.D., D, Sc)
- Breast Carcinoma (MCF-7)
- Prostate Carcinoma (DU-145)
- Colon Carcinoma (HT-29)
- Melanoma (SK-MEL-5)
- Skin Carcinoma (MDA-MB-435)
Inhibition of proliferation of human carcinoma cell lines by phenolic compounds from a Uva - leaf crude extract and its fractions
Phenolic compounds were extracted from the leaves of Uva – bearberry, a potential functional food ingredient, using 80% (v/v) aqueous ethanol after which the resultant crude extract was applied to a Sephadex LH-20 column. A fraction comprising low-molecular-weight phenolics (LMW fraction) and sugars was eluted from the column with 95% (v/v) ethanol. A tannin fraction was then obtained after switching the mobile phase to acetone/water (1:1, v/v).
Phenolic compounds present in the crude extract and its two fractions showed antiproliferative activities in a concentration-dependent manner against five carcinoma cell lines, namely MCF-7 (estrogen receptor-positive breast carcinoma), DU-145 (androgen receptor-negative prostate carcinoma), HT-29 (colon carcinoma), SK-MEL-5 and MDA-MB-435 (melanoma; skin carcinoma).
IC50 data revealed that the tannin fraction was best at retarding cell proliferation in the tested cancer cell lines. The greatest inhibition at 1.5 μg fraction/mL assay was observed for the HT-29 colon carcinoma cell line. The proliferation of SK-MEL-5 skin carcinoma cells was also strongly inhibited by both the crude extract and LMW fraction. MDA-MB-435 cells were found to be the least sensitive for the materials tested, particularly for the LMW fraction. Inhibition of proliferation of human carcinoma cell lines by phenolic compounds from a bearberry-leaf crude extract and its fractions.
The Hidden Truth of Cancer
If the blood develops a more acidic condition, then these excess acidic wastes have to be deposited somewhere in the body. If this unhealthy process continues year after year, these areas steadily increase in acidity and their cells begin to die. Other cells in the affected area may survive by becoming abnormal, these are called malignant. Malignant cells cannot respond to brain commands. They undergo a cellular division that is out of control. This is the beginning of cancer.
Modern medicine treats these malignant cells as if they were bacteria and viruses, it uses chemotherapy, radiation and surgery to treat cancer. Yet none of these treatments will help very much if the environment of the body continues to remain acidic.
However, modern medicine has many paradoxes and dogmas in its theories. These paradoxes are mostly due to the acceptance of the basic concepts of German physicist Virchow’s theory, i.e., Omnis cellula e cellula (all cells come only from cells), and this has been generally believed to be a “golden rule” in the biological and medical sciences.